Background & Aims: Mothers of children with autism face many problems that can affect their life. On the other hand, spirituality, as one of the essential aspects of man’s life, has a significant relationship with human health. This study seeks to determine the spiritual well-being of mothers of children with autism.
Materials & Methods: The type of present study was descriptive-cross-sectional. In this study, mothers were identified among the 7 schools for children with autism, and 200 people participated in the survey in stages, according to the inclusion criteria. The collected data were used using descriptive statistical methods such as setting frequency distribution tables, calculating numerical indices, and independent chi-square and t-test. The paired t-test was used to achieve the goals under SPSS software v. 16.
Results: The Mean±SD spiritual well-being of mothers was 77.17±10.51. Mean±SD spiritual well-being (41.11±5.61) had the highest, and Mean±SD existential well-being (36.06±5.67) had the lowest score. 
Conclusion: In this study, the spiritual well-being dimension of spiritual well-being was in a better position compared to its Existential Dimension of Health. It can be said that the mothers participating in the present study are richer in spiritual well-being compared to existential health.